Systemic Calcium Wave Propagation in Physcomitrella patens

Physcomitrella patens

Arabidopsis and other species, this work has also begun to reveal the levels of evolutionary conservation within the flowering plants and even outside the plant kingdom. What does the future hold for Antirrhinum research? In addition to its continuing role as a genetic model for plant biology and development, several new experimental avenues are beginning to be explored with Antirrhinum. A majo...

UV-A induces two calcium waves in Physcomitrella patens.

Our understanding of the role of Ca2+ in blue/UV-A photoreceptor signaling in a single cell is limited. Insight into calcium signaling has now been attained in Physcomitrella patens and its cryptochrome and phototropin knock-outs. Physcomitrella patens caulonemal filaments grow in the dark by apical extension and their apical cells are highly polarized. Fura-2-dextran ratio images of the apical...

Gene targeting in Physcomitrella patens.

Gene-targeting efficiency in the land plant Physcomitrella patens (Bryophyta) can only be compared with that observed in Saccharomyces cerevisiae. Sequencing programs and microbiological molecular genetic approaches are now being developed to unravel the precise function of plant genes. Physcomitrella patens, as the new 'green yeast', might well become a major tool for functional genomic studie...

II intron in Physcomitrella patens

Culturing the moss Physcomitrella patens.

This article includes a series of methods for culturing the moss Physcomitrella patens at all stages of its life cycle. Gametophytes are axenically cultured on solid agar-based media and in shaken liquid cultures. For long-term storage of gametophytes, cultures are maintained on solid medium at 10°C in a very short day. Cryopreservation may also be used. Finally, sporophytes are generated by se...